Abstract
Trueperella pyogenes (T. pyogenes) is an important opportunistic pathogen in livestock and wild animals. However, only one commercial T. pyogenes vaccine is currently available, and its immunoprotective effect is not ideal. Pyolysin (PLO) is one of the important virulence factors expressed by T. pyogenes and one of the targets for the development of new T. pyogenes vaccines. In this study, we constructed two recombinant antigens, tPLOA1 (contains amino acids 1–110 and domain 4 of the PLO molecule) and tPLOA2 (contains amino acids 190–296 and domain 4 of the PLO molecule). Vaccines were prepared by mixing the two recombinant antigens with incomplete Freund’s adjuvant or sheep red blood cell membrane and provided partial immune protection to immunized mice against the lethal challenge of T. pyogenes. Analysis of the PLO-specific IgG levels of immunized mice indicated that the antibody-inducing potency and immunoprotective efficacy of PLO-based vaccines are affected by the oligomerization and structural changes of PLO after binding to a cholesterol-containing membrane. In addition, the titer of anti-hemolysis antibodies is not a suitable indicator of the immunoprotective effect of these vaccines in PLO-based vaccine-immunized animals. The results provide new insights into the development of T. pyogenes vaccines.
Highlights
The results provide new insights into the development of T. pyogenes vaccines
Trueperella pyogenes (T. pyogenes) is an important opportunistic pathogen in livestock and wild animals, and its infection generally leads to clinical diseases, such as metritis, mastitis, pneumonia, and liver abscess, in economically important animals, such as pigs, cattle, and sheep [1]
T. pyogenes bacterin toxoid and genetic toxoid were widely tested for their potency against T. pyogenes infections or challenge in experimental animals and livestock [6,7,8,9,10]
Summary
Trueperella pyogenes (T. pyogenes) is an important opportunistic pathogen in livestock and wild animals, and its infection generally leads to clinical diseases, such as metritis, mastitis, pneumonia, and liver abscess, in economically important animals, such as pigs, cattle, and sheep [1]. Antibiotics, such as tylosin and tetracycline, are used to prevent liver abscess caused by bacteria such as. Only one vaccine against T. pyogenes is commercially available at present, and its immune protective effect is not satisfactory based on a meeting report of the World Organization for Animal Health (OIE) ad hoc group, which discussed the prioritization of diseases and determined the vaccines that could reduce antimicrobial use in cattle, sheep, and goats
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