Abstract
The human alveolar bone-derived mesenchymal stem cells (hABMSCs) are considered an attractive source for the development of bone tissues. However, their mechanism of action is still unclear. This work aimed to investigate the potential of the natural human growth hormone (NHGH) derived from stem cells under magnetic field (MF) stimulation for tissue engineering by exploring the paracrine or autocrine effects of hABMSCs in vitro. The secretion of anti-inflammatory cytokines and growth factors from hABMSCs was profoundly affected by the intensity of the applied MFs. The effects of stimulated MFs on vascular endothelial growth factor (VEGF) and bone morphogenetic protein-2 (BMP-2) production were quantified by an ELISA kit. Notably, higher cell metabolic activity was observed in MF stimulation compared to the control, and this was more prominent in 130 mT strength of MF. An enhancement in the production of VEGF and BMP-2 was noted in MFs compared to the control. Moreover, higher accumulation of osteogenesis-related genes has occurred in MFs than the control. Furthermore, a significant enhancement in cell metabolic activity and mineralized nodule formations was spotted in the presence of NHGH via MF stimulation; vis-à-vis, MF stimulation only through autocrine and paracrine effects demonstrated the better osteogenic potential of NHGH in the presence of MFs for tissue engineering applications.
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