Abstract
Numbers of functional groups introduced into horseradish peroxidase in reactions with four heterobifunctional reagents, S-acetylmercaptosuccinic anhydride, N-(e-maleimidocaproyloxy)succinimide, N-succinimidyl 3-(2-pyridyldithio)propionate and N-(3-bromoacetamido-n-propionoyloxy)succinimide, were investigated. In the reactions at large molar ratios of the reagents to the enzyme in neutral and slightly alkaline media, maximum numbers of the incorporated functional groups were approximately 2.2 : two reactive amino groups are present in the enzyme molecule.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
