Abstract
Elderflower contains flavonoles, the most important of them being quercetin. These molecules have an important antioxidant effect. The goal of our study was to evaluate which conservation technique of elderflower results the highest concentration of rutin and and phenolic compounds. Further we evaluated the protective effects of these extracts against nephrotoxicity induced by gentamicin in rats. Sambucus nigra extract demonstrates nephroprotective effects in gentamicin-induced renal toxicity, significantly improving outcome and evolution in rats.
Highlights
Elderflower contains flavonoles, the most important of them being quercetin
The main objective of the present study is to evaluate the nephroprotective effect of phenolic compounds found in elderflower inflorescences harvested from local spontaneous flora, in an experimental model of gentamicin induced nephrotoxicity in Wistar rats
Quantitative evaluation of phenolic compounds in elderflowers We obtain a value of rutin quantity from frozen flower of S
Summary
Regarding the determination of phenolic compounds in the N4FU and N4FC study samples (inflorescences without peduncle), it was performed according to the method proposed byMikulic-Petkovsek et al, 2015 [7], with some adjustments. The plasma was separated by centrifugation at 3000 rpm for 10 min at 4°C to determine urea and creatinine concentration. Urine samples were collected by cystocentesis in clear tubes and centrifuged at 1000 rpm for 5 min at 4°C in order to determine the urinary NAG (N-acetyl-beta-D-glucosaminidase) index. Biochemical analysis: creatinine and urea serum concentrations were measured using the spectrophotometric kinetic method and the Jaffe reaction. Determination of urine NAG index: urine samples collected from all subjects were centrifuged at 1000 rpm for 5 min at 4°C the enzyme activity was measured by a spectrophotometric colorimetric method. Statistical values and figures were obtained using GraphPad Prism version 5.0 for Windows, GraphPad Software, San Diego California USA
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