Abstract
To ensure the safety of “Manten-Kirari”, a non-bitter and trace-rutinosidase variety of Tartary buckwheat, we evaluated its mutagenic activity in a bacterial reverse mutagenicity assay, the Ames test. Salmonella typhimurium TA100, TA1535, TA98, TA153, and Escherichia coli WP2 uvrA were employed as test bacteria. The flour of “Manten-Kirari” was dissolved at 12 - 50,000 μg/mL in DMSO and investigated. The number of revertants did not differ compared to the negative control for all concentrations tested, whereas that in the positive control, the number of revertants was increased with or without metabolic activation for each bacterial strain tested. These results suggested that the flour of the Tartary buckwheat “Manten-Kirari” was not genotoxic.
Highlights
Rutin is a flavonoid and is widely distributed throughout the plant kingdom [1]-[4]
The sample was centrifuged at 5000 g, 10 min at 20 ̊C, the resultant supernatant was analyzed using HPLC [17], rutin and quercetin concentrations were determined
We investigated the hydrolysis of rutin by trace amounts of rutinosidase in “Manten-Kirari” flour in DMSO
Summary
Rutin is a flavonoid and is widely distributed throughout the plant kingdom [1]-[4]. Rutin is reported to have various effects such as strengthening the blood capillaries [5] [6], as an antioxidant [7]-[9] and to have alpha*. The clinical effects of rutin in a double-blind crossover study are investigated and reductions in serum myeloperoxidase and cholesterol levels [11], mucosal symptoms, headache, and tiredness are observed [12] For these reasons, attention has been focused on buckwheat as a rutin-rich material for food products [13] [14]. Wilson et al [21] report that intravenous and intraperitoneal injections of 30 to 50 mg/kg in rats and guinea pigs, and intravenous injections of 100 to 200 mg/kg in rabbits, show no deleterious effects Rutin, such as found in “Manten-Kirari”, is not widely consumed in large amounts. We investigate the mutagenic activity of “Manten-Kirari” flour in a bacterial reverse mutagenicity assay (Ames test)
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