Abstract
The purpose of this study is to evaluate the quality of endoscope disinfection at teaching Hospital of Marrakech between March 1st and June 1st, 2015. It is a prospective study of the results of microbiological tests carried out at the teaching Hospital of Marrakech between March 1st and June 1st 2015 over a period of 3 months, with digestive endoscopes. The sampling and the interpretation of the microbiological results were carried out according to the recommendations of the Technical Committee for Nosocomial Infections and Infections Linked to Health Care) (CTINILS). The results obtained showed a residual contamination of total aerobic mesophilic flora (FMAT) at the order of 4,9.103 CFU/ endoscope. 12 microorganisms were isolated and identified. Pseudomonas spp. was the most frequently isolated bacteria in these samples, (47% of all positive samples). The rest is shared between Micrococcus spp (15%), Staphylococcus spp (23%) and Bacillus spp. (10%). The corrective measures established concerning the disinfection procedure adopted in the service made it possible to eradicate the contamination at a target level (FMAT <10 CFU / endoscope). The respect of the basic principles of hospital hygiene and the application of rigorous cleaning and disinfection procedures are major elements in the prevention and fight against nosocomial infections.
Highlights
Digestive endoscopies are diagnostic and therapeutic acts very often performed at the endoscopy unit of the hepatogastroenterology department of the University Hospital of Marrakech
Microbiological contamination of endoscopes was assessed by enumeration of FMAT and by the search for indicator microorganisms including Staphylococcus aureus, Enterobacteriaceae, Pseudomonas aeruginosa and other Pseudomonas, Stenotrophomonas maltophilia, Acinetobacter sp and Candida sp
The results obtained showed a residual contamination of total aerobic mesophilic flora (FMAT) in the order of 4.9 × 10 3 CFU / endoscope. 12 microorganisms were isolated and identified
Summary
Digestive endoscopies are diagnostic and therapeutic acts very often performed at the endoscopy unit of the hepatogastroenterology department of the University Hospital of Marrakech. The number of digestive endoscopies performed exceeds 4964 endoscopies in 2016. The complexity of the structure of endoscopes makes possible an accumulation of organic or mineral stains in certain points, more or less inaccessible to cleaning, disinfection and drying. These soils can themselves contain infectious agents and constitute anchor points for germs and substrates favorable to the multiplication of microorganisms. The infectious risk associated with endoscopy is very real and should never be neglected
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