Evaluation of the mechanisms underlying altered fatty acid biosynthesis in heterotrophic microalgal strain Chlorella sorokiniana during biodegradation of phenol and p-nitrophenol.

Abstract

Phenolic compounds have become a severe environmental concern due to water contamination, affecting the sustainability of the ecosystem. The microalgae enzymes have enticed for the efficient involvement in the biodegradation of phenolics compound in metabolic processes. In this investigation, the oleaginous microalgae Chlorella sorokiniana was cultured heterotrophically under the influence of phenol and p-nitrophenol. The enzymatic assays of algal cell extracts were used to decipher the underlying mechanisms for phenol and p-nitrophenol biodegradation. A reduction of 99.58% and 97.21% in phenol and p-nitrophenol values, respectively, was recorded after the 10th day of microalgae cultivation. Also, the biochemical components in phenol, p-nitrophenol, and control were found to be 39.6 ± 2.3%, 36.7 ± 1.3%, and 30.9 ± 1.8% (total lipids); 27.4 ± 1.4%, 28.3 ± 1.8%, and 19.7 ± 1.5% (total carbohydrates); and 26.7 ± 1.9%, 28.3 ± 1.9%, and 39.9 ± 1.2% (total proteins), respectively. The GC-MS and 1H-NMR spectroscopy attested the incidence of fatty acid methyl esters in the synthesized microalgal biodiesel. The activity of catechol 2,3-dioxygenase and hydroquinone 1,2-dioxygenase in microalgae under heterotrophic conditions has conferred the ortho- and hydroquinone pathways for phenol and p-nitrophenol biodegradation, respectively. Also, the acceleration of fatty acid profiles in microalgae is deliberated under the impact of the phenol and p-nitrophenol biodegradation process. Thus, microalgae enzymes in the metabolic degradation process of phenolic compounds encourage ecosystem sustainability and biodiesel prospects due to the increased lipid profiles of microalgae.