Evaluation of the mechanism of anticancer activity of deltamethrin in Jurkat-J6 cell line

Abstract

Deltamethrin (DLM) is an α-cyano type-II synthetic pyrethroid compound which is extensively used in different agricultural and home pest control. The advantages of pyrethroids over other insecticides are that they are relatively non-toxic to birds and mammals and show high efficacy at relatively lower concentrations. DLM may have dual characteristics i.e. at low molar-concentration, it is nontoxic to normal healthy cells but can induce apoptotic cell death in cancerous cells. There is no reported work based on such hypothesis. Thus, this study has been designed to explore the anticancer property of DLM and the mechanism behind the apoptotic cell death by DLM in cancer cell line (Jurkat J6). Molecular docking study indicates that DLM has the greater binding affinity towards MCL-1 receptor. MTT assay has revealed some significant loss in the viability of cancerous cells by DLM. Further estimation of ROS and GSH have shown the significant oxidative stress induced by DLM in concentration-time dependent manner. DLM has also increased the caspase-3 activity and the apoptotic cells significantly while a decrease in interleukin-2 level has also been observed. The pre-treatment with thiol antioxidant and caspase inhibitor has confirmed the role of oxidative stress and the possibility of other pathways. These observations reveal that DLM may act as anticancer agent at lower concentrations (0.1–1 μM), though the further detailed investigation is warranted.