Abstract
Measurement of neonatal bilirubin using the Jendrassik-Grof method ( x) and the EKTACHEM NBIL assay ( y) was compared over a 6-month period in a total of 1191 specimens from 483 patients less than 30 days of age. Linear regression analysis of the data yields a slope of 0.937, an intercept of 0.387, an S y,x of 0.55 and a correlation coefficient of 0.983 for a total of 1032 specimens from patients ⩽ 14 days of age and a slope of 1.090, an intercept of 0.002, an S y,x of 1.03 and a correlation coefficient of 0.950 for a total of 159 specimens from patients > 14 days of age. The best correlation between EKTACHEM NBIL assay and the reference Jendrassik-Grof method was observed in samples from patients ⩽ 14 days of age. Data from patients older than 14 days showed a higher proportional bias and a lower correlation coefficient between the methods. High performance liquid chromatographic analysis demonstrated that patients > 14 days of age had a higher incidence of elevated β-bilirubin. Linearity extends to 200 mg/l. The NBIL assay provides a rapid, precise micromethod that is less sensitive than the Jendrassik-Grof method to the in vitro photo degradation of bilirubin and is not subject to the interference from hemoglobin and lipids. Because β-bilirubin is not measured by this method, it is only recommended for newborns ⩽ 14 days of age.
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