Evaluation of the involvement of mice (Mus musculus) in the epidemiology of porcine proliferative enteropathy

Abstract

The aim of this study was to evaluate the fecal-oral transmission of L. intracellularis between mice and pigs. The study was divided into two parts. The first part aimed to determine whether mice could be infected by feces from pigs that are experimentally infected with L. intracellularis. Thirty-four Swiss mice received L. intracellularis PCR-positive feces from experimentally infected pigs (M1) for four consecutive days. Twelve other mice received swine negative feces (M2). Pools of mice feces were collected on alternating days post-exposure (dpe). The second part of the study aimed to test whether pigs could be infected when exposed to L. intracellularis PCR-positive feces from experimentally infected mice. Twelve 5-week-old pigs received feed mixed with L. intracellularis PCR-positive mice feces (P1), while the other two pigs received PCR-negative mice feces (P2) for four consecutive days. In the first study, the amount of L. intracellularis provided to M1 boxes per day was between 106 and 108. Mice shed, an average of 104 bacterial units every collection day. Three mice from M1 were positive for L. intracellularis by immunohistochemistry (IHC) at the end of the study. In the second part of the study, pigs in P1 received an average of 105 bacterial units per day. Ten pigs were infected by L. intracellularis based on positive qPCR and/or immunohistochemistry and serology results. These pigs shed an average of 104L. intracellularis/g of feces. Mice and pigs experimentally infected with L. intracellularis can infect each other, therefore, rodents should be considered players in the epidemiology of this disease in pig farms.