Evaluation of the interaction of a guanylhydrazone derivative with cobalt ferrite nanoparticles and PAMAM electrochemical and UV/visible spectroscopic techniques

Abstract

Nowadays, scientific research grows a lot around nanotechnology, which connects many areas of knowledge such as electrochemistry and magnetic nanoparticles (MNPs). The study of bioactive substances by electrochemical methods is already very conceptual, and the association of magnetic nanoparticles has emerged as a new bias of this technique. Here, we report the results of the interaction between the molecule LQM10, a derivative of guanylhydrazone, with nanocarriers: cobalt ferrite magnetic nanoparticles (CoFe2O4 MNPs) and polyamidoamine dendrimer (PAMAM), seeking to unite the characteristics of each nanocarrier to benefit LQM10. The electrochemical system was composed of 3 electrodes, Ag/AgCl/Cl− saturated (reference), platinum (auxiliary), and modified glassy carbon (GCE) (work) with CoFe2O4 MNPs alone and with PAMAM G3, in buffered medium pH 7.03 with or without a co-solvent (analytical grade ethanol). UV-visible spectroscopic studies were carried out in aqueous-ethanolic medium in different concentrations of PAMAM G3 and CoFe2O4 MNPs. The magnetic properties of CoFe2O4 MNPs and each nanocarriers were confirmed by vibrating sample magnetometer and by field effect calorimetry. LQM10, which has a redox profile in oxidative potentials, showed good interaction with all tested electrodes and revealed considerable values of formation constant (KF), highlighting the GCE modified with CoFe2O4 MNPs (KF = 1.92 × 106 L/mol) and PAMAM G3, corroborating with the results of UV-visible data (Kb = 7.60 × 105 L/mol). The generation of heat by magnetic hyperthermia of CoFe2O4 MNPs in the presence of PAMAM G3 and LQM10 was determined (SAR = 0.72 W/g). Therefore, we demonstrated the association of promising nanocarriers (PAMAM G3 and CoFe2O4 MNPs) with anticancer substances and their applicability as magnetic hyperthermia.