Evaluation of the Interaction between Malignant and Normal Human Peripheral Blood Lymphocytes Under Cocultivation and Separate Cultivation

Abstract

Using the Comet assay, the peculiarities of the interaction between malignant and normal human peripheral blood lymphocytes under cocultivation and separate cultivation were investigated. A decrease in Tail Moment was observed against an increase in the frequency of cells in the state of apoptosis in the culture of lymphocytes from conditionally healthy volunteers (bystander cells) under the influence of blood cells from patients with CLL (inductor cells). A statistically significant (p < 0.001) reduction both in the frequency of cells with high levels of DNA damages and apoptotic activity was established in the population of inductor cells under the influence of the bystander cells. The results obtained indicate the realization of both direct (effect of cells-inductors on bystander cells) and rescue (effect of bystander cells on cells-inductors) TIBE phenomenon.