Abstract
Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae), an Old World screwworm fly, is a species with potential for maggot therapy practice and has been described in myiasis and forensic entomology studies. The objective of the present study was to assess the action of different ciprofloxacin concentrations on the growth and development of C. putoria . First instar maggots of the third generation were raised on 60 g of chicken gizzard homogenate in 65% agar diet and received ciprofloxacin chloridrate. Each concentration of the antibiotic tested (3.33 µg/mL, 6.66 µg/mL, and 13.33 µg/mL) and the control (no antibiotic) were replicated four times (40 maggots/replication). The control received distilled water instead of the antibiotic. Maggots were kept in an acclimatized chamber at 30° C during the day and 28° C at night, with 70 + 10% RH and a 14:10 L:D photoperiod. They were weighed in batches of five and stored in test tubes sealed with nylon fabric and elastic. Microsoft Excel and STAT were used for the analysis. The variation among the maggot weight means and the duration of the maggot stage, pupal stage, and time to total development (neolarvae to adult) were analyzed by Student’s t -test (α= 5%). The viabilities and the normality rates were compared using ANOVA, and the expected sex ratio frequency was tested by the chisquared test (χ 2 ). There was no significant difference among the four treatments regarding mean individual maggot weight, mean duration of the maggot inoculation until abandonment, the duration of the maggot and pupal stages, and the total duration of all stages. The sex ratios found in the four treatments did not differ from the expected. Only treatment 2 (6.66 µg/mL concentration of ciprofloxacin) differed significantly from the control in maggot and total viability. The antibiotic did not seem to alter C. putoria development in the postembryonic period.
Highlights
Antimicrobial agents are medications used to eliminate different genera of microorganisms
Control = chicken gizzard homogenate in 65% agar diet; T1 = chicken gizzard homogenate in 65% agar diet + 3.33 μg/mL ciprofloxacin; T2 = chicken gizzard homogenate in 65% agar diet + 6.66 μg/mL ciprofloxacin, T3 = chicken gizzard homogenate in 65% agar diet + 13.33 μg/mL ciprofloxacin
Different concentrations of the same antibiotic need to be tested because the same antimicrobial added to a diet may be safe, inhibitory, or highly damaging depending on the concentration used
Summary
Antimicrobial agents are medications used to eliminate different genera of microorganisms. 2002) and is appropriate for the treatment of infections caused by pathogens sensitive to the drug, such as infections of the upper respiratory tract, ear, nose, throat, maxillofacial area, urinary and kidney tracts, gastrointestinal tract (including typhoid fever), bile duct, soft tissues and infected wounds, bones and joints, gynecological and obstetric infections, septicemia, meningitis, and peritonitis, and can be used to treat imminent risk of infection (prophylaxis) in patients with immune suppression (Quinoflox 2011) Many wounds, such as those mentioned above, can be treated alternatively with the use of maggot therapy, known as maggot debridement therapy, biotherapy, or biosurgery, which consists of applying live fly maggots (Diptera: Calliphoridae) to wounds that do not heal with the objective of removing the necrotic material and promoting new tissue growth. It is important to know how antibiotics used in conjunction with maggots act on the maggots because the antibiotics can influence the natural activities and survival of the maggots
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