Abstract
Measurement of adenosine deaminase (ADA) activity in various biological fluids is useful for the diagnosis of tuberculosis .It is a rapid and noninvasive test unlike the other routine tests used for the diagnosis of tuberculosis especially extra pulmonary tuberculosis. The time limit at which the analysis is done after the sample collection is crucial because the activity of the enzyme can decline with time at ambient temperature. The purpose of the present study is to identify, test, and determine the influence of storage time in the determination of serum adenosine deaminase activity and optimize the time at which the activity of ADA is best, consequently eliminating the error that can occur on delayed testing.. Serum samples collected from 20 healthy individuals both male and females in the age group of 20-60 years was used for the study Serum samples were analyzed on the same day of collection and then stored at -20 0 C and the
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