Evaluation of the Effects of Honey on Acute-Phase Deep Burn Wounds

Yukari Nakajima,Kanae Mukai,Toshio Nakatani,Junko Sugama,Nasruddin Nasruddin,Emi Komatsu,Terumi Iuchi,Yukie Kitayama

doi:10.1155/2013/784959

Yukari Nakajima, Kanae Mukai + Show 6 more

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https://doi.org/10.1155/2013/784959

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Abstract

This study aimed to clarify the effects of honey on acute-phase deep burn wounds. Two deep burn wounds were created on mice which were divided into four groups: no treatment, silver sulfadiazine, manuka honey, and Japanese acacia honey. Wound sizes were calculated as expanded wound areas and sampled 30 minutes and 1–4 days after wounding for histological observation. The wound sections were subjected to hematoxylin and eosin and immunohistological staining to detect necrotic cells, apoptotic cells, neutrophils, and macrophages. The no treatment group formed a scar. The redness around the wound edges in the silver sulfadiazine group was the most intense. All groups exhibited increased wound areas after wounding. The proportions of necrotic cells and the numbers of neutrophils in the manuka and acacia honey groups were lower than those in the no treatment and silver sulfadiazine groups until day 3; however, there were no significant differences between all groups on day 4. These results show that honey treatment on deep burn wounds cannot prevent wound progression. Moreover, comparing our observations with those of Jackson, there are some differences between humans and animals in this regard, and the zone of hyperemia and its surrounding area fall into necrosis, which contributes to burn wound progression.

Highlights

Burns are dynamic injuries that are characterized by their area and depth
This zone corresponds to the zone of stasis because it turned white and fell into necrosis
On day 2, the areas of the wounds continued to increase in all groups, and the new area of expansion revealed a bright white color compared with the zone of coagulation

Summary

Introduction

Burns are dynamic injuries that are characterized by their area and depth. The extent of a burn wound can be calculated by at least 3 different methods: rule of nine, Lund and Browder chart, and palmar surface. Jackson [2] described three concentric zones of burn wound from the intensity of burning and blood flow: the central zone of coagulation, the intermediate zone of stasis, and the outer zone of hyperemia. The white tissue in the zone of coagulation has the greatest direct damage from thermal trauma and is characterized by irreversible necrosis. The zone of coagulation demonstrates complete destruction of the subpapillary vasculature. The intermediate zone of stasis exhibits complete cessation of blood flow within 24 hours and tissue necrosis, and the red and white mottling of the zone of stasis turn white, so the continued tissue necrosis in the zone of stasis contributes to burn wound progression. The zone of hyperemia demonstrates almost complete loss of epidermis without apparent structural damage to the dermis, but capillary loops are patent in the dermis [2, 3]

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