Abstract
The human intestinal microbiota has an important role in the maintenance of human health and disease pathogenesis. The aim of this research was to investigate the impact of four media on human intestinal microbiota metabolite and composition changes, we performed in vitro batch culture using intestinal microbiota samples from three fecal microbiota transplantation (FMT) donors. After 48 h culture, gut microbiota medium (GMM) had the highest production of acetic acid (73.00 ± 7.56 mM) and propionic acid (16.79 ± 1.59 mM), bacterial growth media (BGM) had the highest production of butyric acid (13.39 ± 0.56 mM). In addition, brain heart infusion (BHI) promoted (p < 0.05) the growth of Bacteroidetes, especially Bacteroides after 48 h, GMM resulted in a significant increase (p < 0.05) in Actinobacteria and increased the beneficial genus Bifidobacterium, fastidious anaerobe broth (FAB) increased Firmicutes population, and BGM promoted the growth of Escherichia–Shigella and Akkermansia. The results suggest that four media had different effects on the human intestinal microbiota metabolism and composition in vitro. These results may facilitate the culture of bacteria from the human intestinal microbiota.
Highlights
The human gastrointestinal tract harbors at least 1014 bacterial cells of 400–1000 bacterial species to form the intestinal microbiota that has an important role in the maintenance of human health (Eckburg et al 2005; Tremaroli and Bäckhed 2012)
Comparison of media composition In this study, the human intestinal microbiota from fecal microbiota transplantation (FMT) donors was cultured in four types of media including brain heart infusion (BHI), gut microbiota medium (GMM), fastidious anaerobe broth (FAB) and bacterial growth media (BGM)
The aim of this study was to investigate the impact of four media upon human intestinal microbiota metabolite and composition in anaerobic batch cultures inoculated with human intestinal microbiota samples from FMT donors
Summary
The human gastrointestinal tract harbors at least 1014 bacterial cells of 400–1000 bacterial species to form the intestinal microbiota that has an important role in the maintenance of human health (Eckburg et al 2005; Tremaroli and Bäckhed 2012). Changes in the equilibrium of the gut microbial ecosystem have been associated with a range of diseases, including obesity, type II diabetes and inflammatory bowel disease (Makki et al 2018; Soto et al 2018). Fecal microbiota transplantation (FMT) is a new method to treat a variety of dysbiosis-associated gut diseases, such as Clostridium difficile infection (CDI) (Surawicz et al.2013) and chronic hepatitis B (Ren et al 2017). FMT involves transfer of intestinal microbiota from healthy donors to patients to correct intestinal microbiota dysbiosis (Staley et al 2018). The intestinal microbiota from FMT donors can be used to represent the healthy human intestinal microbiota (Gupta and Khanna 2017)
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