Evaluation of the Effects of Biohcly in an In Vivo Model of Mechanical Wounds in the Rabbit Cornea.

Abstract

Biohcly is a type of acidic nanoclustered water shown to exert an antimicrobial effect and play a role in the tissue-healing process in previous experiments. This study was performed to evaluate the in vivo effects of Biohcly treatment on mechanical corneal wound healing. Rabbit cornea "acute" mechanical wounds were created with an 8-mm hydraulic pressure trephine. The corneal wounds were treated with either Biohcly (left eye) or sterile saline (right eye) 4 times per day. To determine the state of the wounds, the wound healing rate (WHR), inflammatory index (IF), neovascularization, and anterior segment optical coherence tomography (AS-OCT) were evaluated. The expression of inflammatory factors was examined by quantitative real-time reverse transcriptional polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemical staining. Biohcly was more effective than saline in healing corneal ulcers as demonstrated by the WHR calculated on the 9th and 14th days after surgery (P < 0.05). The histological and AS-OCT examinations revealed better regeneration and reduced corneal scars in the Biohcly-treated corneas. No neovascularization formed in the corneas treated with Biohcly, whereas 25% of the saline-treated wounds developed this complication. In addition, the IF scores of the Biohcly-treated wounds were significantly lower than those of the saline-treated wounds. Compared with the right-eye corneas, the left-eye corneas had much lower protein and mRNA levels of matrix metalloproteinase-9 (MMP-9) after the injury. Biohcly plays a role in wound healing and anti-inflammation in the treatment of corneal wounds. The downregulation of MMP-9 in the Biohcly-treated corneas might have been responsible for this effect.