Abstract
The aim the study was to evaluate the effects of autohemotransfusion in adjuvant-induced arthritis model by injections of high and low doses of Complete Freund´s Adjuvant (CFA). Male Holtzman rats (200-230g) were distributed in six groups: control (C); control treated by autohemotransfusion (CT); CFA induced arthritis 0.5% w/v (AIA); CFA induced arthritis 0.5% w/v treated with autohemotransfusion (AIAT); CFA induced arthritis 0.1% w/v (AS) and CFA induced arthritis 0.1% w/v treated with autohemotransfusion (AST). The number of leukocytes, the weight of different organs and the paw volume were analyzed. The autohemotransfusion without erythrocytes promoted a reduction in the number of leukocytes in AIAT and AST when compared to AIA (p < 0.001). In the AST group an increase of the thymus weight (p < 0.05) was observed when compared to C, AIA and AIAT. The autohemotransfusion did not prevent the occurrence of paw edema in arthritic animals of AIAT and AST groups (p>0.05). The autohemotransfusion used in this work presented positive effects on AIA as they promoted a reduction in the number of leukocytes and an increase in thymus weight and body growth. However, other types of autohemotransfusion must be tested to determine the true efficacy of this alternative method of treatment.
Highlights
Rheumatoid arthritis is a chronic inflammatory, multisystemic disease, which affects mainly the joints, it shows autoimmune characteristics and the etiology is unknown
The rats were distributed in six groups: control (C); control treated with autohemotransfusion (CT); Complete Freunds Adjuvant (CFA) induced arthritis 0.5% w/v (AIA); CFA induced arthritis 0.5% w/v treated with autohemotransfusion (AIAT); CFA induced arthritis 0.1% w/v
Missouri, USA) (AS); CFA induced arthritis 0.1% w/v treated with autohemotransfusion (AST)
Summary
Rheumatoid arthritis is a chronic inflammatory, multisystemic disease, which affects mainly the joints, it shows autoimmune characteristics and the etiology is unknown. It is characterized for infiltration and activation of inflammatory cells in tissues and synovial fluid of joints (Odeh, 1997; Kumar, 2007). The joint destruction and perpetuation of immune-mediated chronic inflammation involves the production of proinflammatory substances. These mediators are produced by synovial fibroblasts, monocytes and macrophages stimulated by activated T cells (Choy & Panayi, 2001; Silva, Bersani-Amado, Ishii-Ywamoto, Bracht, & Caparroz-Assef, 2011). The CFA was used to establish the experimental model of induced arthritis in two different concentrations of the bacterial compound, 0.5% w/v (High dose) and 0.1% w/v (low dose) (Donaldson, Seckl & Mcqueen, 1993; Bracht et al, 2012)
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