Abstract
Background. Violation of the regeneration of the tracheal wall after undergoing reconstructive surgeries remains an urgent problem of modern surgery. One of the reasons for the violation of the regeneration processes is insufficient blood supply to the organ. Finding new ways to improve blood flow is a promising area of research. Aim. To evaluate the effectiveness of VEGF effect on the regeneration of the anterior tracheal surface in the early postoperative period. Materials and methods. The study included 60 male Wistar rats at the age of 10 months. All animals underwent tracheal reconstructive surgery; the animals were divided into three groups: group 1 – without medicinal film, group 2 – with prolonged-release medicinal film, group 3 – with prolonged-release medicinal film containing VEGF. The sampling of histological material was carried out on days 3, 7 and 21 of the study, using morphometry to determine the thickness of the tracheal wall, the thickness of the postoperative scar, the area of the surgical intervention zone, the area of the vessels, the ratio of the thickness of the postoperative scar to the wall thickness trachea and the ratio of the area of the vessels to the area of the surgical intervention zone. Results . It was revealed that when exposed to a long-acting medicinal film containing VEGF, significant histological changes occurred on day 7 of the study. In groups 1 and 2, healing occurred by day 21 and was accompanied by defects of the mucous membrane and cartilage. On day 7 in group 3, there was a complete epithelialization of the tracheal wall, an increase in the number of newly formed vessels and glands in the area of surgical intervention. Healing was carried out with a thin postoperative scar without defects in all layers of the tracheal wall. Conclusion . The results obtained indicate the stimulating effect of VEGF on the regeneration of the tracheal wall in the early postoperative period after undergoing reconstructive surgery. The use of VEGF reduces the time of postoperative wound healing and preserves the anatomical structure of the tracheal wall.
Highlights
Violation of the regeneration of the tracheal wall after undergoing reconstructive surgeries remains an urgent problem of modern surgery
Healing of a free tracheal autograft is enhanced by topical vascular endothelial growth factor in an experimental rabbit model
Основы прикладной статистики (использование Excel и Statistica в медицинских исследованиях): учебное пособие
Summary
It was revealed that when exposed to a long-acting medicinal film containing VEGF, significant histological changes occurred on day 7 of the study. Healing was carried out with a thin postoperative scar without defects in all layers of the tracheal wall. The results obtained indicate the stimulating effect of VEGF on the regeneration of the tracheal wall in the early postoperative period after undergoing reconstructive surgery. Нарушение регенерации стенки трахеи после перенесённых реконструктивных операций является актуальной проблемой современной хирургии. Одним из актуальных направлений в профилактике осложнений после перенесённых реконструктивных операций на трахее является разработка новых методов стимуляции регенерации стенки трахеи, направленных на улучшение кровоснабжения в зоне оперативного вмешательства. Данные о роли VEGF в регенерации стенки трахеи противоречивы: ряд авторов отмечают улучшение заживления в зоне реконструктивной операции на трахее, другие описывают разрастание избыточной грануляционной ткани или отсутствие эффекта. В другом исследовании после реконструктивной операции на трахее c использованием аутотрансплантата, обработанного VEGF, отмечалось уменьшение количества грануляционной ткани, фиброза, воспаления и увеличение количества сосудов, а также улучшение процессов заживления. Роль фактора роста эндотелия сосудов в регенерации стенки трахеи в условиях хирургической реконструкции является актуальным вопросом и требует дальнейшего изучения
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