Abstract
BackgroundThis study aimed to investigate the effect of ovariectomy and vitamin D3 on bone microstructure; this effect was examined in three regions of interest at one femoral and two mandibular sampling sites bone in an ovariectomized mouse model.MethodsThirty-six week-old female mice were randomly divided into three groups: 10 subjects were given oral cholecalciferol (vitamin D3) daily for 6 weeks after undergoing bilateral ovariectomy (D3 group), while 10 ovariectomized subjects (OVX) and 10 subjects who underwent a sham operation (SHAM) received peanut oil daily during the investigation. After extermination, the left hemimandible and femur were removed and scanned by micro-CT. The bone micromorphology parameters were analyzed and the BMD was calculated.ResultsThe bone volume fraction (BV/TV) was significantly lower in the trabecular bone of the mandibular condyle in the OVX group than in the SHAM and D3 groups. Also there was a significant difference between the SHAM and D3 groups. The specific bone surface (BS/BV) was significantly higher in the OVX and D3 groups than in the SHAM group. Trabecular thickness (Tb.Th) was significantly higher in the SHAM group, and the trabecular bone pattern factor (Tb.Pf) was significantly higher in the OVX group than in the other two groups. Bone mineral density (BMD) of the femur and the mandible was significantly lower in the OVX group than in the SHAM and D3 groups.ConclusionsOur results show that ovariectomy causes a significantly weaker bone microstructure in the mandibular condyle, where the protective effect of vitamin D3 resulted in a partial resorption.
Highlights
This study aimed to investigate the effect of ovariectomy and vitamin D3 on bone microstructure; this effect was examined in three regions of interest at one femoral and two mandibular sampling sites bone in an ovariectomized mouse model
Thirty-six week-old, 22 g CRL: OF1 (Charles River Laboratories) female mice were randomly divided into three equal groups: 10 subjects were given oral cholecalciferol (4 ng/day Alpha D3-TEVA 0.25 μg) daily for 6 weeks after undergoing bilateral ovariectomy (D3 group), while 10 ovariectomized subjects (OVX group) and 10 subjects after a sham operation (SHAM group) were administered peanut oil daily for 6 weeks during the investigation
In the mandible, the Bone volume fraction (BV/TV) was significantly lower in the trabecular bone of the condyle in the OVX group (44.93% ± 4.09) than in the SHAM (57.61% ± 4.5) and D3 (51.47% ± 5.01) groups; there was a significant difference between the SHAM and D3 groups
Summary
This study aimed to investigate the effect of ovariectomy and vitamin D3 on bone microstructure; this effect was examined in three regions of interest at one femoral and two mandibular sampling sites bone in an ovariectomized mouse model. Osteoporosis affects 200 million people worldwide, and this number is expected to increase dramatically in the future [1]. A frequently used mandibular sampling site is the intermolar spongiosa and—for cortical examination—the base of the mandible. Micromorphological changes in the jaw can significantly influence the progression of periodontal disease or the success of osseointegration around an implant [5]. The effects of drugs used in osteoporosis therapy on the mandibular condyle and condylar cartilage are increasingly under scrutiny [9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
