Evaluation of the effect of two Extraction Methods on the Components of Essential oil of Trifolium pratense L. by GC-MS

Abstract

In order to make full use of Trifolium pratense L. resources, GC-MS was used to evaluate the effects of two extraction methods on the volatile oil components of Trifolium pratense L. A total of 96 compounds were identified by GC-MS from the volatile oil of Trifolium pratense L. extracted by petroleum ether (PE) method and by steam distillation (SD) method. 67 and 42 compounds were identified respectively, with a total peak area of 2.14 × 109 and 1.83 × 108 respectively. The relative percentage of the volatile oil extracted from Trifolium pratense L. by PE method was higher than that of the others, which were Tetracontane (19.70%), Oleic acid (14.21%), 9,12-Octadecadienoic acid (12.51%) and 1 - (+) - Ascorbic acid 2,6-dihydroxadecanoate (12.13%). The relative percentage of the volatile oil distilled from Trifolium pratense L. by SD method were 13 -docosenamide (28.52%), 7,9-di-tert-butyl-1-oxaspiro (4,5) deca-6,9-diene-2,8-dione (3.34%) and L - (+) - ascorbic acid 2,6-dihexadecanoate (2.34%). There were rich compounds in the volatile oil of Trifolium pratense L. Compared with SD method, PE method has higher kinds of compounds and total peak area.