Evaluation of the Effect of Rosa Damascena and Jasminum Sambac on Dental Plaque Regrowth Inhibition

Abstract

Objective: To evaluate the effect of Rosa damascena and Jasminum Sambac on dental plaque regrowth by the Plaque Glycolysis and Regrowth Method (PGRM). Methods: Ethanolic extracts of fresh flowers of Rosa damascena and Jasminum Sambac were prepared and subject to evaluation of antimicrobial property against Streptococcus mutans and also evaluated for their antioxidant property, anti-inflammatory property and their effect on dental plaque regrowth by the PGRM. Results: Both Rosa damascena and Jasminum Sambac showed inhibition of Growth of S. mutans, and MIC for both was found to be 30mg/ml. The antioxidant activity of both showed a dose-dependent increase, with the highest activity of 90.70% at 100 μg/mL concentration for Rosa damascena and 82.93 % at 100 μg/mL concentration for Jasminum Sambac. Both showed some inhibition of RBC haemolysis in the Membrane Protection assay, indicating anti-inflammatory activity, at the concentration of 300 μg/ml. The Percentage RBC Protection for Rosa damascena and Jasminum Sambac was 11.92% and 9.27%. The PGRM assay established that at concentration of 30mg/ml, Extracts of Rosa damascena and Jasminum Sambac showed inhibition of biofilm formation, with highest inhibition observed between 4 hours and 6 hours of incubation, and all were statistically significant. Conclusion: Study concludes that Rosa damascena and Jasminum Sambac have an inhibitory effect on regrowth of dental plaque and has antioxidant, antibacterial and inflammatory properties.