Abstract
Introduction: Cancer is one of the diseases with the highest incidence globally and that associated with the patient's emotional state, can act positively or negatively in the treatment. Cortisol is a principal primary stress hormone in the human body. The corticoids can increase cell proliferation and reactive oxygen species that contribute to DNA damage. Prolonged exposure to stress can contribute to tissues becoming insensitive to cortisol, the primary human stress hormone. Objective: This study explores cortisol's influence on tumor cell development, particularly in human cells of carcinoma of the human laryngeal (HEp-2). Methodology: HEp-2 cells were exposed to increasing cortisol (hydrocortisone) concentrations for 24 or 48 hours, and cytotoxicity (MTT assay) proliferation assay (crystal violet assay), and immunolabeled 3D culture for fibronectin and FAK were analyzed. Results: The group treated with hydrocortisone showed a significant increase in mitochondrial activity, as for the evaluation by the violet crystal, the treated group showed similar behavior to the control. The 3D culture showed dispersed cells within 24 hours with reduced FAK labeling; however, no changes were observed within 48 hours. Conclusion: Although some cases favored corticosteroid use in cancer patients, a more detailed analysis is necessary before prescribing them.
Highlights
Cancer is one of the diseases with the highest incidence globally and that associated with the patient's emotional state, can act positively or negatively in the treatment
Studies relate factors associated with stress to tumor biology; those that describe the effects of glucocorticoids (GC) and their relationship with tumor cells' biology and out (Vitale et al, 2019; Dai et al, 2020; Iftikhar et l., 2021)
2.3 Mitochondrial Metabolic Activity (MTT assay) HEp-2 cells submitted to treatment with hydrocortisone in the periods of 24 and 48 hours were washed with PBS three times incubated with MTT (0.5 mg/ml) for 1 hour at 37°C in an atmosphere of 5% CO2
Summary
Cancer is one of the diseases with the highest incidence globally and that associated with the patient's emotional state, can act positively or negatively in the treatment. Psychological factors can contribute to cancer development given the effects of emotional states on hormonal modification and alter the immune system stress influenced (Reiche et al, 2004; Bedillion et al, 2019). From these possibilities, we increasingly find studies that seek to relate or measure possible influences of psychological and social aspects in the development and potential aggravation of oncological pathologies (Lambert et al, 2020). The dysregulation of cortisol levels, a symptom associated with stress, contributes to the disease process's morbidity, severity, and mortality This alteration includes numerous oncological diseases such as tumor progression in breast cancer (Sephton et al, 2000; Lillberg et al, 2003; Alejandra Ruiz-Manzano et al, 2019)
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