Evaluation of the Dietary Supplementation of a Formulation Containing Ascorbic Acid and a Solid Dispersion of Curcumin with Boric Acid against Salmonella Enteritidis and Necrotic Enteritis in Broiler Chickens.

Daniel Hernandez-Patlan,Ruben Merino-Guzman,Raquel Lopez-Arellano,Billy M Hargis,Mikayla F A Baxter,Abraham Méndez-Albores,Xochitl Hernandez-Velasco,Bruno Solís-Cruz,Karine Patrin Pontin,Juan D Latorre,Guillermo Tellez-Isaias

doi:10.3390/ani9040184

Abstract

Simple SummaryProphylactic or therapeutic administration of a 0.1% mixture containing ascorbic acid (AA) and a solid dispersion of curcumin (CUR) with polyvinylpyrrolidone (PVP) and boric acid (BA) (AA-CUR/PVP-BA) significantly reduced the concentration of Salmonella Enteritidis in broiler chickens and had a positive effect in slightly diminishing the negative impact of necrotic enteritis (NE).Two experiments were conducted to evaluate the effect of the prophylactic or therapeutic administration of a 0.1% mixture containing ascorbic acid and a solid dispersion of curcumin with polyvinylpyrrolidone and boric acid (AA-CUR/PVP-BA) against Salmonella Enteritidis (S. Enteritidis) in broiler chickens. A third experiment was conducted to evaluate the impact of the dietary administration of 0.1% AA-CUR/PVP-BA in a necrotic enteritis (NE) model in broiler chickens. The prophylactic administration of 0.1% AA-CUR/PVP-BA significantly decreased S. Enteritidis colonization in cecal tonsils (CT) when compared to the positive control group (PC, p < 0.05). The therapeutic administration of 0.1% AA-CUR/PVP-BA significantly reduced the concentration of S. Enteritidis by 2.05 and 2.71 log in crop and CT, respectively, when compared with the PC on day 10 post-S. Enteritidis challenge. Furthermore, the serum FITC-d concentration and total intestinal IgA levels were also significantly lower in chickens that received 0.1% AA-CUR/PVP-BA. Contrary, the PC group showed significantly higher total intestinal IgA levels compared to the negative control or AA-CUR/PVP-BA groups in the NE model. However, 0.1% AA-CUR/PVP-BA showed a better effect in reducing the concentration of S. Enteritidis when compared to the NE model. Further studies with higher concentration of AA-CUR/PVP-BA into the feed to extend these preliminary results are currently being evaluated.

Highlights

Enteric bacterial pathogens pose a threat to intestinal health and can contribute to the transmission of zoonotic diseases [1,2], increased mortality in poultry flocks, reduced feed efficiency, decreased rate of body weight gain and, increase in total production costs [3,4]
The ascorbic acid (AA)-CUR/PVP-boric acid (BA) mixture was mixed into the feed for 15 min using a rotary mixer to obtain the experimental diet with a final concentration of 0.1% (1 g/kg of feed)
Enteritidis samples in cecal tonsils (CT) compared to Positive control group (PC)

Summary

Introduction

Enteric bacterial pathogens pose a threat to intestinal health and can contribute to the transmission of zoonotic diseases [1,2], increased mortality in poultry flocks, reduced feed efficiency, decreased rate of body weight gain and, increase in total production costs [3,4].Salmonella infection and necrotic enteritis (NE) produced by Clostridium perfringens (CP) are two significant bacterial diseases in poultry [5,6]. Enteric bacterial pathogens pose a threat to intestinal health and can contribute to the transmission of zoonotic diseases [1,2], increased mortality in poultry flocks, reduced feed efficiency, decreased rate of body weight gain and, increase in total production costs [3,4]. It has been reported that the presence of salmonellosis has caused significant economic losses in poultry production due to the reduction in overall performance and high mortality in affected flocks [4,9]. Another economically significant disease affecting chicken production is NE induced by CP and occurs in two forms. In its acute clinical form, NE can cause significant flock mortality [10,11,12]

Methods

Results

Conclusion