Evaluation of the Correlation between the rs4918 Polymorphism of AHSG Gene and Coronary Artery Calcification in Patients with Coronary Artery Disease

Zeynab Ahmadihosseini,Morteza Moeinian,Sepideh Elyasi,Saeed Nazemi,Amir Hooshang Mohammadpour

doi:10.3390/cardiogenetics10020007

Zeynab Ahmadihosseini, Morteza Moeinian + Show 3 more

Open Access

https://doi.org/10.3390/cardiogenetics10020007

Copy DOI

Abstract

Objectives: Fetuin-A is a circulating calcification inhibitor that prevents coronary artery calcification (CAC) by increasing calcium phosphate solubility and inhibiting VSMC differentiation and apoptosis. In this study, we investigated the correlation between rs4918 and CAC in patients with coronary artery disease (CAD). Methods: Forty-two healthy individuals and eighty-one CAD patients were recruited in the present study. The CAC score (CACS) was measured by CT angiography and the genotype analysis of rs4918 single-nucleotide polymorphism SNP was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Results: The CACS was significantly higher in CAD patients compared to healthy individuals (p < 0.001); however, there was no significant difference between the mean CACS in the presence and absence of rs4918 (p = 0.792). The mean calcium score of the left main coronary artery (LMCA) was significantly lower in carriers of the rs4918 allele (p = 0.036). The frequency of rs4918 SNP was almost similar in the control group and CAD patients (p = 0.846). Conclusions: in patients with CAD, we found no significant association between rs4918 SNP and CACS, indicating that carriers of this allele are not at increased risk of developing cardiovascular diseases compared with those without.

Highlights

Coronary artery disease (CAD) is one of the major causes of mortality worldwide, and atherosclerosis is the primary etiology [1]
The coronary artery calcium score was significantly higher in CAD patients compared to healthy individuals (p < 0.001); no significant difference was observed in the CAC score (CACS) in the presence and absence of rs4918 single-nucleotide polymorphism (SNP) (p = 0.792)
Coronary artery calcification has been considered as a non-traditional risk factor of cardiovascular disorders and events

Summary

Introduction

Coronary artery disease (CAD) is one of the major causes of mortality worldwide, and atherosclerosis is the primary etiology [1]. Coronary artery calcification (CAC) is the characteristic feature of atheroma plaque, and its extent is increased with the progression of the lesion. Calcified plaques are associated with an almost 1.7-fold higher incidence of mortality, independent of other cardiovascular risk factors [2]. It is believed that the transdifferentiation of VSMCs to the osteoblastic lineage and the loss of balance between inhibitors and inducers of calcification are the prominent etiologies of ectopic calcification. In this context, fetuin-A has been identified as an important inhibitor of ectopic calcification

Objectives

Methods

Results

Conclusion