Abstract
The development of effective cleaning products to remove microbial loads has been of extreme importance for the Sterilization Centers of Dental-Medical-Hospital Materials. The addition of enzymes to these products has promoted a beneficial effect, which efficiency depended on the biofilm and the enzymes evaluated. Thus, the objective of this work was to analyze the efficiency of such cleaning agents that contain enzymes of the protease class in the removal of bacterial biofilm of E. coli under different conditions of time, temperature and pH.
Highlights
One of the major health care challenge in hospital infections is to get an efficient way to remove dirtiness and bacterial biofilms from medical devices avoiding high costs for the health care system[1]
The objective of this work was to evaluate the efficiency of these cleaning agents containing enzymes of the protease class in the removal of the bacterial biofilm of E. coli under different conditions of time, temperature and pH
Escherichia coli ATCC 35218 biofilm was developed in BHI medium for 24h at 37°C in 96-well plates for further analysis of biofilm removal[2]
Summary
One of the major health care challenge in hospital infections is to get an efficient way to remove dirtiness and bacterial biofilms from medical devices avoiding high costs for the health care system[1]. Corrosive chemicals and high temperatures can not be used for this purpose due to the delicacy of some medical devices, constituting a great difficulty of cleaning in materials centers and sterilization. Softer solutions, such as enzymatic detergents, must be carefully developed to ensure effective performance.
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