Evaluation of the Bystander effect caused ultrasound waves on the MCF-7 cell line

Abstract

Introduction: Non-target radiation effects are damages and effects that occur without the need for direct radiation exposure in cells. Bystander signals cause non-targeted irradiation effect that has been defined as radiation responses in which non-irradiated cells exhibit irradiated effects as a result of signals from adjacent irradiated cells. In this study, the bystander effects of ultrasound radiation on the MCF-7 cell line have been investigated. Materials and Methods: Two main groups were defined as target group and bystander group. Target group contained 3 subgroups. The first subgroup is control group which has no intervention. The second subgroup was exposed to ultrasound with the frequency of 1MHZ and intensity of 0.5 . The Third subgroup was exposed to ultrasound with the frequency of 1MHZ and intensity of 1 . The bystander group has 3 subgroups, too. Bystander subgroups received cell culture medium of target subgroups, respectively. The MTT assay has been investigated for bystander subgroups. Results: The MTT assay results showed cell viability in bystander cells that received the culture medium from irradiated target cells has not been reduced. In other words, no significant difference was observed between first and second bystander subgroups with control group (P > 0.05). Conclusion: Ultrasound waves with intensity of 0.5 and 1 do not induce the bystander effect on MCF-7 cell line.