Evaluation of the bone tissue mechanical parameters after induced alimentary Cu-deficiency followed by supplementary injection of Cu nanoparticles in rats

Abstract

The paper studies the evaluation of mechanical properties of bone tissue with Cu- deficiency in the body. The studies have been conducted on the bones of rats subjected to a complete Cu-deficient diet and the Cu-deficient diet, followed by rehabilitation diet. Within the frames of research testing on bending of animals’ femoral bones was conducted, the scheme for flexure of the axes "move-force" midsection was drawn. The Young's modulus, ultimate normal and tangential stress limits were defined. The animals under research were divided into three groups: a) with Cu-deficiency, b) Cu-deficiency within 5 weeks with the following rehabilitation, c) Cu-deficiency within 8 weeks with the following rehabilitation. Depending on time and Cu-deficiency in the body the analysis of the obtained mechanical characteristics was performed. The effect of Cu-deficiency on bone tissues’ rigidity and strength was detected. It was also shown that Cu-deficiency within 5 weeks and further rehabilitation the mechanic properties of the bone tissues are restored and the hardening analogue takes place. The recovery does not take place at the Cu-deficiency within 8 weeks, what the authors connect with the tissue texture transformation.