Abstract
ss Open Acce Poster Presentation Evaluation of the baculovirus and E.coli-expressed non-structural (NS) proteins of bluetongue virus (BTV) as antigen in an indirect or competition ELISA to differentiate infected from vaccinated animals Lissette Lopez*1, Angel Venteo1, Miguel Angel Jimenez-Clavero2, Juan Luis Carrasco1, Maria Jose Cano1, Elena Soria1, Concepcion GomezTejedor2, Antonio Sanz1, Carmen Vela1 and Paloma Rueda1
Highlights
bluetongue virus (BTV) is an important disease of wild and domestic animals both from the aspect of animal health as well as economical impact
The protein was purified by guanidine chloride 6 M and a further step by immobilized metal affinity chromatography (IMAC) (Ni2+)
In the case of the baculovirus expression system, the gene was cloned into the pAcHLT-A transfer vector which added a poly-His tag at the N-terminus
Summary
Evaluation of the baculovirus and E.coli-expressed non-structural (NS) proteins of bluetongue virus (BTV) as antigen in an indirect or competition ELISA to differentiate infected from vaccinated animals. Lissette López*1, Angel Venteo, Miguel Angel Jiménez-Clavero, Juan Luis Carrasco, María José Cano, Elena Soria, Concepción Gómez-. 28037 Madrid, Spain and 2Laboratorio Central de Veterinaria. The 4th Recombinant Protein Production Meeting: a comparative view on host physiology The organisers would like to thank Novozymes Delta Ltd who generously supported the meeting. Meeting abstracts – A single PDF containing all abstracts in this supplement is available here http://www.biomedcentral.com/content/pdf/1475-2859-5-S1-info.pdf
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
