Evaluation of the Association of Omentin 1 rs2274907 A>T and rs2274908 G>A Gene Polymorphisms with Coronary Artery Disease in Indian Population: A Case Control Study.

Chandan K Jha,Shaheena Banu,Abdullatif Taha Babakr,Imadeldin Elfaki,Jamsheed Javid,Rashid Mir,S M S Chahal

doi:10.3390/jpm9020030

Abstract

Coronary artery disease (CAD) is a major cause of death all over the world. CAD is caused by atherosclerosis which is induced by the interaction of genetic factors and environmental factors. Traditional environmental risk factors include hyperlipidemia, diabetes mellitus, lack of exercise, obesity, poor diet and others. Genome-wide association studies have revealed the association of certain gene polymorphisms with susceptibility to CAD. Omentin 1 is an adipokine secreted by the visceral adipose tissues and has been reported to have anti-inflammatory, cardioprotective, and enhances insulin sensitivity. In this study, we examined the role of omentin-1 common single nucleotide polymorphisms (SNPs) (rs2274907 A>T and rs2274908 G>A) in CAD. We genotyped 100 CAD patients and 100 matched healthy controls from the south Indian population using an amplification refractory mutation system (ARMS-PCR) and allele-specific PCR (AS-PCR). Our result indicated the rs2274908 G>A is not associated with CAD. Results showed that there was a significant difference in rs2274907 A>T genotype distribution between controls and CAD cases (P-value < 0.05). Results indicated that the AT genotype of the rs2274907 is associated with CAD with OR = 3.0 (95% confidence interval (CI), 1.64 to 5.49), 1.65 (1.27 to 2.163), P = 0.002. The T allele of the rs2274907 was also associated with CAD with OR = 1.82 (95% CI, 1.193 to 2.80), 1.37 (1.08 to 1.74), P = 0.005. Rs2274907 genotype distribution was also correlated with serum total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), hypertension and diabetes. We conclude that the AT genotype and the T allele of the rs2274907 A>T is associated with Cad in the south Indian population. Further studies on the effect of the rs2274907 A>T on omentin-1 function are recommended, and future well-designed studies with larger sample sizes and in different populations are required to validate our findings.

Highlights

Cardiovascular diseases (CVDs) are one of the major causes of hospitalization and death and account for about 31%of deaths worldwide In majorIndia, causes about 25%of the deaths and are death causedand by Cardiovascular diseases (CVDs) are one [1].of the of hospitalizationCVDs, and about of CVD deaths occur prior to the age of years, while in Europe about account for about 31% of deaths worldwide [1]
CVDs are an important cause of death and morbidity caused mainly by atherosclerosis [20]
Macrophage phagocytes lipoprotein and forms the foam cells, which is a characteristic of the fatty streak phase of atherosclerosis [21,22]

Summary

Introduction

CVDs, and about of CVD deaths occur prior to the age of years, while in Europe about account for about 31% of deaths worldwide [1]. Of CVD productive lives [2].CVDs. CVDs arethe a Indian group population of pathological that years include deaths occuryears beforeof thistheir age [2], affect in the conditions most productive of atherosclerosis, coronary artery disease (CAD), angina, myocardial infarction, rheumatic and their lives [2]. CVDs are a group of pathological conditions that include atherosclerosis, coronary artery congenital heartangina, diseases, stroke andinfarction, venous thromboembolism [3]. Traditional risk factors for CVD disease (CAD), myocardial rheumatic and congenital heart diseases, stroke and include age, low high-density lipoprotein cholesterol (HDL-C), high low-density lipoprotein venous thromboembolism [3]. Traditional risk factors for CVD include age, low high-density lipoprotein cholesterol (HDL-C),

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