Evaluation of the association between dilated cardiomyopathy and single nucleotide polymorphisms of the lamin A/C gene in the Iranian population

Abstract

IntroductionSingle nucleotide polymorphisms (SNPs) play a significant role in the development of dilated cardiomyopathy (DCM). In the present study, the association between DCM and SNPs of the lamin A/C (LMNA) gene in the Iranian population were evaluated. MethodBetween Feb to Dec 2020, 30 samples of DCM and 30 samples of healthy individuals were obtained, of which all these 66 individuals referred to Labbafinejad, Imam Hossein and Masih Daneshvari hospitals, Tehran, Iran. Five mL of the peripheral blood of the participants were used for DNA extraction. Then, the amplified fragments of DNAs, which were done by polymerase chain reaction (PCR), were sent to Macrogen (South Korea) for sequencing via the Sanger sequencing method. ResultsThe results of alignment of PCR product sequences showed that there was no significant relationship between mutation in the LMNA gene, and sex plus ethnicity of DCM patients (P > 0.05). In addition, significant connection between mutation in L3, L4 and L5 locations of rs57077886 and rs28933090 and sex of the patients, was not observed too. ConclusionIn conclusion, however, previous surveys showed that there was a significant correlation between LMNA gene mutation and DCM, but present study shows that it's not true all the time. This matter should be taken into consideration in longitudinal research.