Abstract
To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis. Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p<0.05). Enemas containing sucralfate improves the inflammation and increases the tissue contents of neutral and acid mucins. The content of neutral mucins does not change with the time or concentration of sucralfate used, while acid mucins increases with concentration and time of intervention. Sucralfate enemas improve the inflammatory process and increase the tissue content of neutral and acid mucins in colon without fecal stream.
Highlights
Glotzer et al.[1] were the first to describe the development of an inflammatory process in the mucosa of colon segments without fecal stream
The most common histological finds in patients with diversion colitis (DC) include epithelial erosions or ulcerations, decreased length or modified architecture of the colonic glands, inflammatory infiltrate and, depletion of the number of goblet cells, with consequent modifications on tissue content of mucins2-4These histological alterations are similar to those found in other forms of inflammatory bowel disease (IBD), in the distal proctitis founded after pelvic radiotherapy and in rectal compromising of ulcerative colitis (UC)
We found a significant increase in tissue content of neutral mucins in animals subjected to irrigation with SCF compared to animals irrigated with S.F. 0.9%, regardless of the concentration and duration of intervention
Summary
Glotzer et al.[1] were the first to describe the development of an inflammatory process in the mucosa of colon segments without fecal stream. They called this new form of inflammatory bowel disease (IBD) diversion colitis (DC). The proportions between neutral and acid mucins may be modified in IBD and changes to the tissue expression of this glycoprotein in the colon mucosa of patients with UC and Crohn’s disease have already been demonstrated[11,12]. Recent studies have shown the importance of maintaining the supply of SCFAs, butyrate, in order to have adequate gene expression relating to mucin formation and content of the glycoprotein[4,5,8,13]
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