Abstract
The API-ZYM system (Analytab Products) was evaluated for potential use for confirming the identity of Listeria isolates. A total of 68 Listeria strains from various sources were assayed using standard procedures. All isolates were positive for esterase (C4), esterase lipase (C8), phosphohydrolase (one exception), β-glucosidase, acid phosphatase and lipase (one exception). Negative responses were observed consistently for valine aminopeptidase, trypsin, α-galactosidase, β-galactosidase, β-glucuronidase, α-fucosidase and cystine aminopeptidase (one exception). Variable responses were noted for alkaline phosphatase, leucine aminopeptidase, chymotrypsin, α-glulucosidase and N-acetyl glucosaminidase. Comparison of these results with those of other Gram positive species indicated that the API-ZYM system could be used for rapid confirmation of Listeria to the subgenus level.
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