Evaluation of the antioxidant potential of in vitro propagated hyssop (Hyssopus officinalis L.) with different plant growth regulators

Abstract

An efficient method for the micro propagation of hyssop (Hyssopus officinalis L.) has been developed. The plants were cultured on Murashige and Skoog's medium supplemented with 6-benzyl-aminopurine, thidiazuron, zeatin and indole-3butyric acid after four weeks of culture. Maximum numbers of shoots, shoot length, fresh weight and dry weight were established at 1.0 mg/L 6-benzyl-aminopurine and 0.1 mg/L indole-3-butyric acid. For induction of root, uniform micro shoots were excised and transferred to the rooting medium (half strength Murashige and Skoog medium macro and microelements) supplemented with 0.1 mg/L indole-3-butyric acid and 20 g/L sucrose. Indole-3-butyric acid increased culture rooting, number of roots and root length more efficiently at 0.1 mg/L after four weeks of culture. The multiple plants were successfully ex vitro adapted with 90% survival. The described protocol allows the establishment of numerous micro propagated plants of H. officinalis. The used growth regulators for hyssop micro propagation influenced plant antioxidant system. Antioxidant defence of micropropagated H. officinalis determined by the activities of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase) resulted in higher shoot formation and increasing of shoot number per explant.