Evaluation of the Antioxidant and Inhibitory Activity of Aloe vera plant Extract (Fermented by Lactobacillus plantarum bacteria) Against the Pathogenic Bacteria Isolated from Diarrhea Infections

Abstract

The current study aimed to isolate bacterial species from people suffering from diarrhea, diagnose them, and prepare the aqueous extract of Aloe vera plant, and ferment it by the locally-isolated Lactobacillus plantarum bacteria, and determining the presence of phenolic compounds before and after fermentation.The antioxidant activity was estimated using the FRAP method and the inhibitory activity against bacterial isolates causing diarrhea was also determined. The results showed that the pathogenic bacterial species causing diarrhea were Salmonella, E. coli. Shigella and Proteus.The lactobacillus bacteria isolated and diagnosed from local dairy samples was Lactobacillus plantarum that was used to ferment Aloe vera plant extract. The types of phenolic compounds identified and estimated in Aloe Vera extract were Chlorogenic acid, Caffiec acid, Cinnamic acid, Gallic acid, Coumaric acid, Vitexin and Syringic acid.The highest antioxidant activity was (48), which was found to be after fermentation, compared to its value before fermentation, which was (39). It was also shown that the highest inhibitory activity efficiency of the fermented Aloe vera extract was against pathogenic isolates from Non-fermented extrac