Abstract
Purpose: To evaluate the antioxidant effect of 80 % ethanol extract and its n-hexane and dichloromethane fractions of Ya-hom Intajak, and its 47 medicinal plants components.Methods: The 80 % ethanol extract and its n-hexane and dichloromethane fractions were investigated on human low-density lipoprotein (LDL) peroxidation by thiobarbituric acid reactive substances (TBARS) assay. Antioxidant effect was also determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and ferric reducing antioxidant power (FRAP) assays.Results: The extract of Ya-hom Intajak exhibited moderate DPPH scavenging activity with an IC50 value of 99.08 µg/ml and a FRAP value of 1.12 mmol FeSO4/g. Among the individual plants, Terminalia chebula Retz., Caesalpinia sappan L., Cinnamomum bejolghota (Buch.-Ham.) and Ci. verum J. Presl, showed strong antioxidant activities. T. chebula was the most potent plant in the DPPH assay while Ca. sappan had the highest FRAP value. Among the extracts, the dichloromethane fraction showed the highest inhibitory effect on LDL peroxidation with a half maximal inhibitory concentration (IC50) value comparable to that of probucol (0.82 µg/ml). Pearson correlation analysis revealed that total phenolic content (TPC) showed high positive correlations with FRAP (r = 0.908, p < 0.01) and DPPH activities (r= 0.648, p < 0.01).Conclusion: Ya-hom Intajak is a valuable source of natural antioxidants and has a potential application as a cardiovascular protective formulation.Keywords: Ya-hom Intajak, Antioxidant activity, Terminalia chebula Retz., Caesalpinia sappan L., Cinnamomum bejolghota (Buch.-Ham.), Ci. verum J. Presl , Phenolic content
Highlights
The initiation and progression of early stage of atherosclerosis and the development of cardiovascular diseases have been attributed to the oxidation of low-density lipoproteins (LDL)
DPPH radical scavenging capacity and ferric reducing antioxidant power (FRAP) have been used to evaluate antioxidant activity of the ethanol extract of Yahom Intajak and its fraction together with 47 medicinal plants contained in the Ya-hom formula
The samples were evaluated for their radical scavenging capacity using DPPH assay which is based on the scavenging of the stable DPPH by an antioxidant
Summary
The initiation and progression of early stage of atherosclerosis and the development of cardiovascular diseases have been attributed to the oxidation of low-density lipoproteins (LDL). Many antioxidants have been developed to delay or inhibit the oxidation of the biomolecules by terminating the initiation or propagation of the oxidizing chain reactions by free radicals and inhibiting the foam cell formation [1]. The antioxidant activity of phenolic compounds is mainly due to their redox properties, which play an important role in adsorbing and neutralizing free radicals, quenching singlet and triplet oxygen or decomposing peroxides [2]. The bioactive components can effectively inhibit LDL oxidation and may prevent atherosclerosis by reducing and slowing down the progression to advance stage [5]
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