Evaluation of the anti-microbial activity of various concentration of wheat grass (Triticum aestivum) extract against Gram-positive bacteria: An in vitro study

Abstract

Introduction: The increases in multidrug-resistant pathogens has become a challenge to the researcher to develop newer natural and active components to combat these problems in future. Hence this study was undertaken to determine the anti-microbial activity of wheat grass ( Triticum aestivum ) extract against Gram-positive bacteria. Materials and Methods: Experimental design, in vitro study, lab setting. Wheat grass powder ( T. aestivum ) was obtained from the pharmacy in Belgaum city. Ethanolic extract of wheat grass powder was prepared by cold extract method. The extract was then diluted by inert solvent, dimethyl formaldehyde, to obtain different concentration of extract. The extract, along with the concentrations of range 1-10, was then subjected to microbiological investigation to determine which concentration of the extract will give a wider inhibition zone against Streptococcus mutans and Lactobacillus spp. Results: Minimum inhibitory concentration by broth dilution method was found to be 5% of extract for S. mutans and 1.25% for Lactobacillus spp. Conclusion: Wheat grass extract demonstrated anti-microbial activity against S. mutans and Lactobacillus spp.