Evaluation of the Antigenotoxic Potential of Methanolic Leaves Extract of Triticum aestivum in Mice

Amit Nayak,Gaurav Jain,Govind Nayak,Shailesh M Kewatker

doi:10.9734/jpri/2021/v33i47b33088

Amit Nayak, Gaurav Jain + Show 2 more

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https://doi.org/10.9734/jpri/2021/v33i47b33088

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Abstract

Mutations are changes to the nucleotide sequence of the genetic material of an organism. Reactive oxygen species (ROS) play an important role in process like mutagenesis, carcinogenesis and aging by their ability to damage cellular DNA. Inhibition of mutagenesis or carcinogenesis is generally not based on one specific mechanism. Protection against cancer can occur at different stages of the complicated processes of carcinogenesis. Naturally occurring antioxidants have been extensively studied for their capacity to protect organisms and cells from oxidation. Triticum aestivum (T. aestivum) have revealed its medicinal potential for some human diseases; therefore, this study aimed to evaluate the genotoxic and antigenotoxic potential of methanolic extract. To accomplish this, the methanolic extract of T. aestivum was evaluated for its antigenotoxic effect using the chromosomal aberrations and micronucleus assay of bone marrow cells of mice.T. aestivum extract has shown significant protection against Cyclophosphamide induced micronucleus formation and chromosomal aberrations.

Highlights

Oxidative deoxy ribonucleic acid (DNA) damage is caused by mutagen which leads to mutagenesis
It is undertaken for two main reasons: a) to detect chemicals that might cause genetic damage, including point mutations in germ cells and increase the burden of genetic disease in the human population and b) to detect chemicals that might be carcinogenic [13,14]
Polychromatic erythrocytes (PCE), non-chromatic erythrocytes (NCE) and micronucleus (MN) are the forms of micronucleus formation produced by administration of cyclophosphamide in the bone marrow cells of mice given in Table 1 and Figs. 1-2

Summary

Introduction

Oxidative deoxy ribonucleic acid (DNA) damage is caused by mutagen which leads to mutagenesis. It is crucial step in the development of cancer and other degenerative processes such as cardiovascular and neurodegenerative disease as well as premature ageing [1]. Genotoxicity testing is a significant part of the threat estimation of chemicals for regulatory purposes [11,12] It is undertaken for two main reasons: a) to detect chemicals that might cause genetic damage, including point mutations in germ cells and increase the burden of genetic disease in the human population and b) to detect chemicals that might be carcinogenic (based on the assumption that a mutagenesis is a key event in the process of carcinogenesis) [13,14]. In the past few years, there has been considerable interest in natural products endowed with antimutagenic and anticarcinogenic properties

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