Evaluation of the Anticonvulsant Activity of the Hydroethanolic Extract of Crinum scillifolium Bulbs (Amaryllidaceae) in Experimental Animals

Koua Kadio Brou Donald,Kouakou Gisèle Siransy,Yapi Houphouet Felix,Yapo Adou Francis,Miezan Bile Aka Patrice,Kouakou Sylvain Landry,N'Tamon Amon Diane Marina

doi:10.13189/app.2017.050202

Koua Kadio Brou Donald, Kouakou Gisèle Siransy + Show 5 more

Open Access

https://doi.org/10.13189/app.2017.050202

Copy DOI

Abstract

Crinum species is frequently used for the treatment of nervous disorders such as epilepsy. This study aimed to investigate the anticonvulsant activity of the hydroethanolic extract of the Crinum scillifolium bulbs in chemoconvulsant-induced seizures in mice. The anticonvulsant activity of the extract (25, 50, 100, 200 and 400mg/kg), was investigated in isoniazid (INH)-induced seizures in mice. The hydroethanolic extract protected mice from INH-induced seizures in a dose-dependent manner. 100% of protection was observed when the animals were treated with 200 and 400mg/kg of hydroethanolic extract of Crinum scillifolium. At Dose of 50 and 100mg/kg 83% protection was observed; the onset of convulsion significant was delayed, and no mortality was found of the mice against isoniazid-induced convulsion. Mice pretreated with hydroethanolic extract at the dose of 25mg/kg reduced mortality to 27% and significant delayed the onset of death (p<0.01). In conclusion, Crinum scillifolium was revealed possessing anticonvulsant effects in mice, via the GABAergic neurotransmission.

Highlights

Epilepsy is a chronic brain disorder characterized by recurrent derangement of the nervous system due to sudden excessive disorderly discharge from the cerebral neurons
Group 1 received respective distilled water (10 ml/kg p.o) ; group 2 was allotted for standard drug and group 3, 4, 5, 6 and 7 received graded doses of hydroethanolic extract of Crinum scillifolium (25, 50, 100, 200 and 400mg/kg; p.o)
To the reference drug clonazepam, the extract protected the animals with 100% of protection, when was treated with 200 and 400mg/kg of hydroethanolic extract of Crinum scillifolium

Summary

Introduction

Epilepsy is a chronic brain disorder characterized by recurrent derangement of the nervous system due to sudden excessive disorderly discharge from the cerebral neurons. It is one of the most common serious disorders of the brain, affecting some 50 million persons worldwide [1]. The prevalence of epilepsy in developed countries is estimated to 5‰ and Africa 10‰ [3]. World Health Organization estimated that approximately 80% people with epilepsy live in developing countries and most of them do not get adequate medical treatment [5] Current epilepsy therapy with modern anticonvulsants is associated with side effect, chronic toxicity as well as teratogenic effects limit their use and 30% of patients continue to have seizures with current anticonvulsant therapies [4].

Objectives

Methods

Results

Conclusion