Evaluation of the Antibacterial Effects of Four Different Adhesives Against Three Bacterial Species in Two Time Periods: An In Vitro Comparative Study

Abstract

Aim: The study aimed at evaluating the antibacterial activities of four adhesive systems against three bacterial species in two time periods. Material and Method: Four different antibacterial adhesive materials, a nonantibacterial adhesive (Clearfil SE Bond), and a vancomycin-impregnated antibiogram disc were used in the study. The antibacterial activities of Gluma 2 Bond, Clearfil SE Protect Bond (CPB) Primer, FL Bond II (FLB), and Peak Universal Bond (PUB) on Enterococcus faecalis, Streptococcus mutans, and Lactobacillus acidophilus were evaluated by the disc diffusion method. Antibiogram discs onto which the adhesive agent was dripped were placed on agar plates at intervals. Inhibition zone diameters around the discs, which were incubated, were measured. Statistical analysis was done with the one-way analysis of variance. Results: As a result of our study, it was concluded that the CPB Primer was the agent with the highest antibacterial activity, including the control group. One of the remarkable findings in our study was that CPB Primer showed the most significant effect against Streptococcus mutans among microorganisms. Although not statistically significant, the antibacterial effect of adhesive agents at the end of the 48th h was found to be higher than at the end of the 24th h ( P > .05). Conclusion: The strong antibacterial activity of the CPB Primer on all three bacteria suggests that it is a preferable adhesive agent in deep dentinal caries as well as in initial caries lesions.