Evaluation of the Anti-inflammatory, Antimicrobial, Antioxidant, and Cytotoxic Effects of Chitosan Thiocolchicoside-Lauric Acid Nanogel.

Abstract

The present study explored the anti-inflammatory, antimicrobial, antioxidant, and cytotoxic effects of a combination of chitosan thiocolchicoside and lauric acid (CTLA) nanogel. Materials and methods: A nanogel formulation of thiocolchicoside and lauric acid was developed and tested for potential applications. The antimicrobial activity was assessed using the well diffusion method, while the antioxidant activity was evaluated using the 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging assay and hydrogen peroxide (H2O2) antioxidant assay methods. The anti-inflammatory activity was determined through the egg albumin denaturation method, the bovine serum albumin denaturation method, and the membrane stabilization assay. A brine shrimp lethality assay was used to study the cytotoxic effect of the nanogel. We identified significant positive outcomes for the CTLAnanogel. The results showed a percentage of inhibition of 81% at 50μg/mL, which showed the nanogel's significant anti-inflammatory activity by inhibiting bovine serum albumin denaturation. The anti-inflammatory properties of the nanogel were comparable to the standard diclofenac sodium at all tested concentrations. The egg albumin denaturation assay results revealed a percentage inhibition of 76% at 50 μg/mL. In the membrane stabilization assay, a percentage inhibition of86%was obtained at a concentration of 50 μg/mL against 89% for the standard drug. The nanogel exhibited a zone of inhibition of 20 mm against Streptococcus mutans and 22 mm with a dilution of 100 µg/mL of CTLA nanogel against Staphylococcus aureus. The antioxidant activity was studied by using the DPPH method, 50 μg/ml has an 89% inhibition, which was similar to the standard. The inhibitory activity of CTLAnanogel at 50 μg/ml was81.6%in the hydroxyl free radical scavenging assay, which was comparable to the standard drug. At 5 μg/mL concentration of CTLA nanogel, approximately 90% of the nauplii remained alive after 48 hours. The CTLAnanogel showed excellent anti-inflammatory and antioxidant properties suggesting its potential for managing inflammatory conditions and oxidative stress-related disorders.