Abstract
BackgroundCedrus deodara (Roxb.) Loud (normally called as deodar), one out of four species in the genus Cedrus, exhibits widely biological activities. The Cedrus deodara total lignans from the pine needles (CTL) were extracted. The aim of the study was to investigate the anticancer potential of the CTL on A549 cell line.MethodsWe extracted the CTL by ethanol and assessed the cytotoxicity by CCK-8 method. Cell cycle and apoptosis were detected by a FACS Verse Calibur flow cytometry.ResultsThe CTL were extracted by means of ethanol hot refluxing and the content of total lignans in CTL was about 55.77%. By the CCK-8 assays, CTL inhibited the growth of A549 cells in a dose-dependent fashion, with the IC50 values of 39.82 ± 1.74 μg/mL. CTL also inhibited the growth to a less extent in HeLa, HepG2, MKN28 and HT-29 cells.ConclusionAt low doses, the CTL effectively inhibited the growth of A549 cells. By comparison of IC50 values, we found that A549 cells might be more sensitive to the treatment with CTL. In addition, CTL were also able to increase the population of A549 cells in G2/M phase and the percentage of apoptotic A549 cells. CTL may have therapeutic potential in lung adenocarcinoma cancer by regulating cell cycle and apoptosis.
Highlights
Cedrus deodara (Roxb.) Loud, one out of four species in the genus Cedrus, exhibits widely biological activities
We systematically investigated the effects of CTL on the proliferation of cancer cells in vitro and revealed a pronounced change in the cell cycle and apoptosis that was possibly required for CTL to exert anti-cancer mechanism
We found that the content of total lignans content in CTL was about 55.77% by using this method
Summary
Cedrus deodara (Roxb.) Loud (normally called as deodar), one out of four species in the genus Cedrus, exhibits widely biological activities. CD has been described to display therapeutic effects in expelling wind, destroying parasites, removing dampness and relieving itches in the Dictionary of Chinese Crude Drugs It has been widely utilized in Chinese drinks and has been. As is case for CD, these chemical compounds exhibit therapeutic effects against inflammation, pain, spasm, diabetes, Herpes simplex virus type-1 and cancer [5, 6]. Their antivirus and antibacterial effects have been reported [7, 8].
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