Abstract
Population aging is a prominent global problem in today’s society. However, there are currently no good methods to treat or prevent aging, so anti-aging research has crucial implications. In this research, we screened bacteria from centenarians, and finally selected four probiotics (Lactobacillus fermentum SX-0718, L. casei SX-1107, Bifidobacterium longum SX-1326, and B. animalis SX-0582) to form a probiotic combination. By using the senescence accelerated mouse prone 8 (SAMP8) model, the anti-aging effects of the probiotic combination were evaluated by using behavioural testing, neuroinflammation, intestinal inflammation, and intestinal microbiota. The results showed that probiotic combination improved the impaired spatial memory, motor dysfunction, and decreased exploratory behavior in aging mice. The probiotic combination inhibited Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NFκB)-induced neuroinflammation and up-regulated the expression of Sirt 1 to protect hippocampal neurons. At the same time, the probiotic combination regulated the intestinal microbiota, reduced the relative abundance of Alistipes and Prevotella in SAMP8 mice, inhibited TLR4/NFκB-induced intestinal inflammation, and increased the expression of intestinal permeability related proteins zonula occludens-1 (ZO-1) and Occuldin. The anti-aging effects of the probiotic combination may be through the regulating intestinal microbiota and inhibiting TLR4/NFκB-induced inflammation. This research provides the basis and technical support for the future production and application of the probiotic combination.
Highlights
Aging is a process that almost all living organisms go through, characterised by the gradual decline of in the body’s cell, tissue, and organ functions over time, as well as reduced cognitive and memory functions [1]
The selected probiotics significantly inhibited the growth of the pathogens S. typhimurium ATCC 13311, Sh. flexneri ATCC 12022, P. acnes ATCC 11827, Sh. dysenteriae 301, E. coli O157, S. enteritidis ATCC 13076, L. monocytogenes ATCC 19111, Staph. aureus Cowan1 and C. albicans SC531, with a suppression area from 15.25 mm to 22.5 mm (Figure 1C)
The detection of inflammatory factor protein levels showed that probiotic combination significantly reduced the relative expression of the pro-inflammatory cytokine IL-1b, IL6 and tumour necrosis factor-a (TNF-a) compared with the M group, (Figure 4B; p
Summary
Aging is a process that almost all living organisms go through, characterised by the gradual decline of in the body’s cell, tissue, and organ functions over time, as well as reduced cognitive and memory functions [1]. Anti-Aging Effects of a Probiotic Combination diseases - Parkinson’s disease (PD), Alzheimer’s disease (AD), malignant tumours, and others – continues to rise, causing a heavy financial burden on the country and the families of patients [3, 4]. Growth factors, proteases, and other substances that cause inflammation and destroy the cellular microenvironment, leading to reduced cell survival. It affects the proliferation and differentiation of cells and induces stem cell senescence and aging-related diseases [9]. Bruunsgaard et al [11] found that the increased of TNF-a is correlated positively with the all-cause mortality of elderly men in a follow-up study of 333 relatively healthy elderly people over 80 years of age, indicating that TNF-a has a certain predictive effect on death
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