Evaluation of the Angiogenic Activity of Salvia triloba L. Essential Oil

Abstract

The genus Salvia L. (Lamiaceae) is represented by 89 species, thereof forty five endemic in Turkey [1]. Most of the Salvia species are used in various preparations and forms including the essential oil, in folk medicine among other uses for their anti-inflammatory, antipyretic, pain relieving and wound healing properties [1,2]. In this study, the herbal parts of S. triloba obtained from a commercial source cultivated in Izmir, Turkey, was investigated both for its (anti-)angiogenic properties and for its essential oil composition. The essential oil was obtained by hydrodistillation, which was analyzed both by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Main constituents were identified as 1,8-cineole (44%), camphor (12%), α-pinene (6%), β-pinene (6%), camphene (5%), and myrcene (3%). Using the in vivo CAM (Chorio Allantoic Membrane) assay the Salvia essential oil and its main constituents (0.5–100 µg/pellet) as well as in vitro cytotoxicity (MTT), cell migration and tube formation tests (HUV-EC-C cell lines) of the essential oil (0.01–200 µM) in comparison with standards such as suramin, thalidomide, cortisone were investigated for their angiogenic properties. As a result, S. triloba essential oil showed in both tests antiangiogenic activity in a dose dependent manner. Acknowledgements: TUBITAK – SBAG-107S262 (3756) for financial support.