Evaluation of the age and anatomical areas impact on the properties of canine adipose-derived multipotent mesenchymal stromal cells

Abstract

Multipotent mesenchymal stem cells (MMSCs) are potentially promising cells for non-pharmacological therapy. We have carried out a comparative analysis of MMSCs populations isolated from different anatomical areas of canine adipose-derived tissue (subcutaneous and visceral adipose tissue) with different age (one year and ten years) characterized by immunophenotype CD44 + / CD90 + / CD105 + / CD45-. Histochemical analysis revealed that the number of cells in per unit volume of canine adipose tissue decreases with age. The number of cells derived of stromal-vascular fraction by filtration method (filters 80 microns) also revealed the dependence on the age and anatomical location of the collected adipose tissue. In dogs of one year age, the number of cells in this population was 2.4 ≥ times more than in the experimental groups of 10 years. The necessity of collection adipose tissue from dogs of 10 years for further isolation of MMSCs has been justified, compared with dogs aged one year.