Abstract
Porodaedalea pini (Brot.) Murrill. a member of the family Hymenochaetaceae and is a highly admirable traditional mushroom for the treatment of various diseases and disorders. The present study aimed to investigate the microscopic and macroscopic features, phytochemical analysis, and antioxidant potential of different extracts of Porodaedalea pini by using the 2, 2-diphenyl-1-picryhydrazyl, ferric reduction antioxidant potential assay, total antioxidant capacity, total phenolic content, and total flavonoid content. The DNA nicking assay and polyphenolic detection were carried out for the most potent fraction. After the complete study, it was concluded that the methanolic extract has shown higher antioxidant activity as compared to the hexane, ethyl acetate and chloroform extracts. In the plasmid DNA nicking assay, the methanolic extract has shown a concentration-dependent increase in protective activity. The UHPLC profiling of methanol extract for 12 different standard polyphenolic compounds has shown the presence of all compounds except rutin, ellagic acid, and kaempferol. The presence of these polyphenols might be responsible for its antioxidant activity.
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