Abstract
ABSTRACTExperimental leishmaniasis is widely used to study the effector functions of T helper cell subsets in vivo. Healing and nonhealing Leishmania major infections have been correlated with T helper 1 and T helper 2 responses, respectively. In the present study, we determined T cell effector functions ex vivo, without any further restimulation and compared them to those obtained following antigen‐specific restimulation in vitro. Our results show that T helper cell responses are significantly less polarised when determined ex vivo as compared to those measured after restimulation in vitro. Moreover, the differences in CD4+ T cell proliferation observed between healer and nonhealer strains of mice differed ex vivo and in vitro. Our results suggest that determination of both ex vivo as well as in vitro T cell responses are crucial to characterise immune responses during experimental leishmaniasis.
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