Evaluation of SRAP markers for mapping of Pisum sativum L.

María Fernanda Guindon,Aldana Zayas,Vanina Cravero,Eugenia Martin,Enrique Cointry

doi:10.1590/1984-70332016v16n3a28

Abstract

Linkage maps have become important tools for genetic studies. With the aim of evaluating the SRAP (sequence-related amplified polymorphism) technique for linkage mapping in Pisum sativum L., a F2 mapping population derived from an initial cross between cvs. DDR11 and Zav25 was generated. A total of 25 SRAP primer combinations were evaluated in 45 F2 plants and both parental lines, generating 208 polymorphic bands/markers. The markers were analyzed by the chi-square goodness-of-fit test to check the expected Mendelian segregation ratio. The resulting linkage map consists of 112 genetic markers distributed in 7 linkage groups (LGs), covering a total of 528.8 cM. The length of the LGs ranged from 47.6 to 144.3 cM (mean 75.54 cM), with 9 to 34 markers. The linkage map developed in this study indicates that the SRAP marker system could be applied to mapping studies of pea.

Highlights

Pea (Pisum sativum L.) is an autogamous, annual cool-season legume originated from areas in the Middle East, in the East of the Caucasus, Iran and Afghanistan, and West of the Mediterranean basin (Smýkal et al 2011)
We proposed the use of the sequence-related amplified polymorphism (SRAP) technique (Li and Quiros 2001) to generate a number of markers distributed across all pea chromosomes
SRAPs were established as a powerful tool for construction of genetic linkage maps, e.g., of Brassica (Li and Quiros 2001, Sun et al 2007), Gossypium (Lin et al 2005, Yu et al 2007), Cucumis melo L. (Wang et al 2008), and more recently of Cynara cardunculus (Martin et al 2013)

Summary

Introduction

Pea (Pisum sativum L.) is an autogamous, annual cool-season legume originated from areas in the Middle East, in the East of the Caucasus, Iran and Afghanistan, and West of the Mediterranean basin (Smýkal et al 2011). Peas were an important source of animal and human food for many centuries. The global dry pea production averages 10 million tonnes a year. The rising world population will require increased crop production. Some researchers suggest that the current rate of increase in crop yields will not be enough to meet this demand (Tester and Langridge 2010). Plant breeding programs are needed to further raise crop yields. In this context, linkage mapping will be useful to maximize the success probability. Genetic linkage maps are powerful tools for genetic research and breeding of plants. Different kinds of markers, such as simple sequence repeats (SSR; Loridon et al 2005), single nucleotide polymorphisms (SNP; Deulvot et al 2010), inter simple sequence repeats (ISSR; Mishra et al 2009), and sequence tagged sites

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Conclusion