Abstract
Erythrolamprus poecilogyrus sublineatus (Cope, 1860), is a species widely distributed in the Pampa Domain, occurring in Rio Grande do Sul, Argentina and Uruguay, mainlyin the pampa region. In the coastal region of southern Brazil this is serpent is considered one of the most abundant. The purpose of the present study is to describe the techniques of sperm evaluation in vitro for E. poecilogyrus sublineatus in the coastal plain of Rio Grande do Sul, Brazil. After laparatomy the efferent vases were collected and the semen was diluted in 1ml Beltsville Thawing Solution. The characteristics of motility, membrane integrity, mitochondria, acrosome, DNA, cell viability and cellular functionality were evaluated. Fluorescent probes were used for the evaluation of sperm structure in epifluorescence microscope. With the techniques described, it was possible to identify intact and injured cells, enabling the determination of cell characteristics for the spring season (October and November). It was observed in the analyses that 80% of sperm cells were mobile and that 84.1 ± 8.0% of sperm membranes were intact. The standards found were of 48 ± 13.8% of intact acrosome, 73.6 ± 6.0 of perfect DNA and of 91.8 ± 4.0 of functional mitochondria. Thus, these values from the sperm analysis can be used as standards for the species Erythrolamprus poecilogyrus sublineatus.
Highlights
The biggest obstacle for the success in reproduction of wild animals in captivity is the lack of knowledge of basic information about most species, including aspects on their reproductive biology (Swanson, 2006)
In Brazil 245 species are recorded, representing almost 75% of snake species that occur in the country (Bérnils and Costa, 2012)
The objective of the study is to evaluate the ejaculation of E. poecilogyrus sublineatus according to the technical standards of sperm in vitro evaluation
Summary
The biggest obstacle for the success in reproduction of wild animals in captivity is the lack of knowledge of basic information about most species, including aspects on their reproductive biology (Swanson, 2006). Assisted reproduction in reptiles is still underused, due to the little information about the methods of collecting, spermatic evaluation and cellular structure or artificial insemination (Mattson et al, 2007; Zacariotti et al, 2007). Dipsadidae comprises the largest group of living snakes, with more than 1,700 living species distributed in all continents except the Antarctic. This family inhabits all terrestrial environments and substrates (Lema, 2002). In Brazil 245 species are recorded, representing almost 75% of snake species that occur in the country (Bérnils and Costa, 2012)
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Schedule a callMore From: Brazilian journal of biology = Revista brasleira de biologia
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