Abstract
More specific antigens are still being searched for in order to increase informativeness of techniques for early diagnostics of tuberculous infection. Aimed at the investigation of proteins of tuberculous mycobacteria (PPD-L, ESAT 6, 85, hybrid of ЕSAT 6 CFP 10, CFP32B, Rv2660c) the study was conducted including testing of induced IFN-γ in the children ill with tuberculosis in the early period of primary tuberculous infection and also those not infected with tuberculous mycobacteria – 130 children in the age from 6.0 ± 0.4 years old. It was found out that tuberculin remained to be valuable for evaluation of the intensity of anti-tuberculosis immunity. Antigens of ESAT 6, 85, CFP32B, Rv2660c were identified as proteins of the early stage of tuberculous infection. The presence of response when stimulating by the above antigens was typical of the favorable course of tuberculosis. Antigens of ESAT 6 and Rv2660c were valuable for assessment of latent tuberculous infections and the hybrid of ЕSAT 6 CFP 10 – for diagnostics of tuberculosis. Therefore introduction of immunological tests (in vitro) into the set of diagnostic tools allows optimizing the early diagnostics of tuberculous infection in children and forecasting the course of the disease when tuberculosis has developed.
Highlights
Для повышения информативности методов ранней диагностики туберкулезной инфекции продолжаются поиски более специфичных антигенов
С целью оценки белков м икобактерий туберкулеза (М Б Т ) (П П Д -Л, ESAT 6, 85, гибрид ESAT 6 C F P 10, C F P 32B, R v2660c) проведено исследование, включающее определение индуцированного И Ф Н -у у детей, больных туберкулезом, в раннем периоде первичной туберкулезной и нф екции, а такж е не и н ф иц и рованн ыхМБТ - 130 детей в возрасте 6,0 ± 0,4 года
Антигены ESAT 6, 85, CFP32B, Rv2660c определили как белки ранней стадии туберкулезной инфекции
Summary
Антигены ESAT 6, 85, CFP32B, Rv2660c определили как белки ранней стадии туберкулезной инфекции. Антигены ESAT 6 и Rv2660c являлись значимыми в оценке латентной туберкулезной инфекции, а гибрид ESAT 6 C F P 10 - в диагностике туберкулеза. EVALUATION OF SPECIFIC ANTIGENS AT THE EARLY STAGE OF TUBERCULOUS INFECTION IN CHILDREN. Низкая информативность специфических проб для диагностики латентной туберкулезной ин ф екци и у детей [3, 5] требует поиска более ч у в ствительных антигенов, в том числе полученных генно-инж енерным путем [6]. Определение недавно идентифицированных белков микобактерий (C F P 32B, Rv2660c) может быть перспективным для диа гностики латентной туберкулезной инфекции у детей. Цель исследования: оценка влияния белков ми кобактерий туберкулеза на уровень индуцирован ного гамма-интерферона у детей с туберкулезной инфекцией
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